Journal: The AAPS Journal

Article Title: Comparison of Neutralizing Antibody Assays for Receptor Binding and Enzyme Activity of the Enzyme Replacement Therapeutic Naglazyme(R) (Galsulfase)

doi: 10.1208/s12248-008-9048-1

Figure Lengend Snippet: Schematic of enzyme activity neutralizing antibody assay. The total antibody fraction is isolated from the Protein A/G step and preincubated with rhASB prior to addition of a fluorogenic substrate 4-MUS. Enzyme activity is measured by release of 4-MU from the cleavage of 4-MUS. Percent inhibition is evaluated relative to enzyme activity with antibodies isolated from a pool of naïve serum

Article Snippet: After comparing capacity and reproducibility of various resin and bead systems, the UltraLink Protein A/G resin was selected for implementation. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. 6 caption a7 Schematic of enzyme activity neutralizing antibody assay.

Techniques: Activity Assay, Isolation, Inhibition